Behind every diagnosis is accurate laboratory testing. In the Medical Lab Assistant / Technician Diploma Program, you’ll learn the scientific techniques and laboratory procedures that support physicians and healthcare teams in delivering patient care.
About the Medical Lab Assistant /Technician Diploma Program
Accurate laboratory testing plays a critical role in modern healthcare. The Medical Laboratory Assistant / Technician Diploma Program prepares you with the practical laboratory skills and scientific knowledge needed to support diagnostic testing and help healthcare teams deliver effective patient care.
Students develop expertise in specimen collection, laboratory safety, medical terminology, and diagnostic testing procedures while building the precision and professionalism required in clinical environments. Through hands-on labs and real-world clinical placements, you’ll gain experience working with the equipment and processes used in medical laboratories every day.
The program also prepares graduates to challenge the Medical Laboratory Professionals’ Association of Ontario (MLPAO) certification exam and pursue careers supporting physicians and healthcare teams.
Accredited with Condition
The Trillium College Medical Lab Assistant/Technician programs at our Oshawa, Kingston, and St. Catharines campuses have successfully moved to the next step in accreditation. These programs are currently Accredited with Conditions.
Admitted with Equal
Trillium College is happy to announce that the Medical Lab Assistant/Technician program is now open for enrollment at our Sudbury campus. The Sudbury Campus is now Admitted with Equal.
Course Overview
Working in a medical laboratory requires precision, technical skill, and a strong understanding of laboratory procedures. The Medical Laboratory Assistant / Technician Diploma Program prepares students with the practical training and scientific knowledge needed to support diagnostic testing in modern healthcare environments.
Throughout the program, students develop hands-on laboratory skills including specimen collection, specimen handling and processing, laboratory safety procedures, medical terminology, and diagnostic testing practices. This training helps students build the accuracy, attention to detail, and professionalism required when working with patient samples and laboratory equipment.
The program is recognized by the Medical Laboratory Professionals’ Association of Ontario (MLPAO), and graduates gain the prerequisites needed to apply for the MLPAO certification exam, an important step toward provincial certification and professional recognition within the field.
Graduates are also prepared to challenge the Canadian Society for Medical Laboratory Science (CSMLS) national certification exam. These pathways provide graduates with recognized credentials that support career opportunities in medical laboratories and healthcare settings across Ontario and beyond.
The program has also progressed through the accreditation process and is currently Accredited with Conditions at the Oshawa, Kingston, and St. Catharines campuses, with the Sudbury campus Admitted with EQual. These milestones reflect Trillium College’s commitment to quality education and continuous improvement while ensuring training aligns with industry expectations.
Admission Requirements
Ontario Secondary School Diploma or Mature Student Status
Trillium College Immunization Report; completed and signed by a medical professional.
Submission of a clear Police Check (CPIC) report with Vulnerable Sector Screening
Graduation Requirements
220 Placement hours completed
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A Curriculum that Transforms
Working in a medical laboratory requires precision, technical skill, and a strong understanding of professional responsibility. Throughout the Medical Laboratory Assistant / Technician Diploma Program, you’ll develop the laboratory techniques and clinical knowledge needed to support diagnostic testing and contribute to patient care as part of a healthcare team.
Students learn how to collect, handle, and process medical samples while working alongside physicians, registered laboratory technologists, and other healthcare professionals. Training is delivered through live instructor-led virtual lectures and in-person laboratory training, allowing you to build both the scientific knowledge and practical skills required in modern medical labs.
Key areas of training include:
Specimen Collection & Laboratory Skills
Learn how to safely collect, label, transport, and process blood and other medical specimens using proper laboratory procedures.
Specimen Collection & Laboratory Skills
Learn how to safely collect, label, transport, and process blood and other medical specimens using proper laboratory procedures..
Laboratory Safety & Infection Control
Develop safe laboratory practices that protect patients, staff, and the integrity of diagnostic testing.
Laboratory Safety & Infection Control
Develop safe laboratory practices that protect patients, staff, and the integrity of diagnostic testing..
Terminology & Documentation
Build the communication and record-keeping skills required in healthcare environments.
Terminology & Documentation
Build the communication and record-keeping skills required in healthcare environments..
Hematology & Phlebotomy
Develop precision in blood collection techniques and laboratory testing procedures.
Hematology & Phlebotomy
Develop precision in blood collection techniques and laboratory testing procedures..
Microbiology & Professional Ethics
Study laboratory science while building the ethical judgment and professionalism expected in healthcare settings.
Microbiology & Professional Ethics
Study laboratory science while building the ethical judgment and professionalism expected in healthcare settings..
Specimen Collection & Laboratory Skills
Learn how to safely collect, label, transport, and process blood and other medical specimens using proper laboratory procedures.
Specimen Collection & Laboratory Skills
Learn how to safely collect, label, transport, and process blood and other medical specimens using proper laboratory procedures..
Laboratory Safety & Infection Control
Develop safe laboratory practices that protect patients, staff, and the integrity of diagnostic testing.
Laboratory Safety & Infection Control
Develop safe laboratory practices that protect patients, staff, and the integrity of diagnostic testing..
Terminology & Documentation
Build the communication and record-keeping skills required in healthcare environments.
Terminology & Documentation
Build the communication and record-keeping skills required in healthcare environments..
Hematology & Phlebotomy
Develop precision in blood collection techniques and laboratory testing procedures.
Hematology & Phlebotomy
Develop precision in blood collection techniques and laboratory testing procedures..
Microbiology & Professional Ethics
Study laboratory science while building the ethical judgment and professionalism expected in healthcare settings.
Microbiology & Professional Ethics
Study laboratory science while building the ethical judgment and professionalism expected in healthcare settings..
Hands-on learning is central to the program. Through in-person labs and 100 hours of volunteer experience, students practice real-world laboratory procedures—including specimen collection, processing, and analysis—under the supervision of experienced instructors. This practical training builds confidence, supports preparation for the MLPAO certification exam and the Canadian Society for Medical Laboratory Science (CSMLS) national certification exam., and ensures graduates are ready to enter medical laboratory environments.
Why Study to Become a Medical Lab Assistant /Technician at Trillium College?
Choosing the right training program is an important step toward building a healthcare career. The Medical Laboratory Assistant / Technician Diploma Program combines hands-on laboratory training, clinical experience, and MLPAO-recognized education to prepare students for professional practice in medical laboratory environments.
Students learn from experienced instructors while developing real-world skills through in-person labs and supervised training. Live, instructor-led virtual classes provide flexibility while allowing students to stay engaged with instructors and classmates throughout the program.
Graduates are prepared to challenge professional certification exams, including the Medical Laboratory Professionals’ Association of Ontario (MLPAO) certification exam and the Canadian Society for Medical Laboratory Science (CSMLS) national certification exam. These pathways provide graduates with recognized credentials that support career opportunities in medical laboratories and healthcare settings across Ontario and beyond.
Learn from Industry Experts
Accelerated Programs
Instructor-led Virtual Learning
Year-Round Enrollments
Career Coaches and Advisors
Student Financial Planners
Learn from Industry Experts
Accelerated Programs
Instructor-led Virtual Learning
Year-Round Enrollments
Career Coaches and Advisors
Student Financial Planners
A Career Full of Possibilities
Graduates of the Medical Laboratory Assistant / Technician Diploma Program step into an essential support role within the healthcare system. By collecting, preparing, and processing medical specimens, you help ensure laboratory results are accurate and available to healthcare professionals who rely on them to support patient diagnosis and treatment.
Your training can lead to opportunities in a variety of healthcare and laboratory environments, including:
*Salaries are based on 2026 data. Please refer to the provided link for the latest updates.
A Career Full of Possibilities
Graduates of the Medical Laboratory Assistant / Technician Diploma Program step into an essential support role within the healthcare system. By collecting, preparing, and processing medical specimens, you help ensure laboratory results are accurate and available to healthcare professionals who rely on them to support patient diagnosis and treatment.
Your training can lead to opportunities in a variety of healthcare and laboratory environments, including:
*Salaries are based on 2026 data. Please refer to the provided link for the latest updates.
Are You Ready to Start College?
You've explored the program and thought about your future. Now take one more Step - see how ready you really are for college life!
Take the test in minutes
Answer a quick series of questions that assess how ready you are for college by exploring your personality, goals, and potential roadblocks to success.
Get personalised results
Build a customized snapshot of your strengths, goals, and any roadblocks that may affect your college journey—so you know exactly where you stand.
Connect with a career coach for guidance
Walk through your results with a Career Coach who will help you discuss your goals, address any roadblocks, and decide whether the program is the right fit for you.
You've explored the program and thought about your future. Now take one more Step - see how ready you really are for college life!
Take the test in minutes
Answer a quick series of questions that assess how ready you are for college by exploring your personality, goals, and potential roadblocks to success.
Get personalised results
Build a customized snapshot of your strengths, goals, and any roadblocks that may affect your college journey—so you know exactly where you stand.
Connect with a career coach for guidance
Walk through your results with a Career Coach who will help you discuss your goals, address any roadblocks, and decide whether the program is the right fit for you.
Hear From Our Students
I found that Trillium eased their students into all of the processes at the right pace. I would absolutely recommend Trillium. They will work with you closely to make sure that you succeed more than any other school I have experienced or looked at before.
AlyshaMedical Lab Assistant/Technician Student
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I found that Trillium eased their students into all of the processes at the right pace. I would absolutely recommend Trillium. They will work with you closely to make sure that you succeed more than any other school I have experienced or looked at before.
Alysha
Medical Lab Assistant/Technician Student
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Medical Lab Assistant /Technician FAQs
The Medical Laboratory Assistant / Technician Diploma Program prepares students for careers supporting diagnostic testing in healthcare environments. Students receive hands-on training in specimen collection, phlebotomy, laboratory safety, and specimen processing while developing the technical and professional skills required in modern medical laboratories.
The hybrid program is available at our Oshawa, Kingston, Niagara, and Sudbury campuses. Students attend live instructor-led virtual lectures online while completing in-person laboratory sessions on campus, allowing them to build both theoretical knowledge and hands-on laboratory skills.
The MLAT program is delivered in a hybrid format, combining live virtual lectures with hands-on laboratory training on campus. This approach allows students to build the theoretical knowledge required for laboratory work while developing the practical skills needed in clinical environments.
Students develop expertise in several key areas of laboratory practice, including:
• Specimen collection and laboratory operations
• Venipuncture, phlebotomy, and hematology
• Clinical microbiology and chemistry for diagnostic testing
• Medical terminology and laboratory documentation
• Professional ethics and communication in healthcare settings
A hands-on labs and 220 hours of clinical placement provide practical training that helps prepare graduates for employment and professional certification.
Yes. The program includes phlebotomy and venipuncture training, allowing students to develop the skills needed to safely collect blood specimens from patients.
Students practice proper specimen collection techniques, patient identification procedures, infection control, and safe handling of laboratory samples through hands-on labs and supervised training.
The Medical Laboratory Assistant / Technician Diploma Program is recognized by the Medical Laboratory Professionals’ Association of Ontario (MLPAO). The program has also progressed through the accreditation process and is currently Accredited with Conditions at the Oshawa, Kingston, and St. Catharines campuses, with the Sudbury campus registered Admitted with EQual.
This recognition helps ensure the program meets industry standards and prepares graduates to pursue certification and employment in hospitals, clinics, and medical laboratories.
Learn more: Medical Laboratory Professionals’ Association of Ontario (MLPAO)
To become a certified Medical Laboratory Assistant / Technician, you must complete training and successfully pass a recognized certification exam.
The typical pathway includes:
• Complete the Medical Laboratory Assistant / Technician Diploma Program at Trillium College.
• Finish all required coursework, labs, and volunteer experience to build practical laboratory skills.
• Apply to write a recognized certification exam. Graduates of the program are eligible to challenge two certification
options:
o MLPAO Certification – the primary certification used by many employers in Ontario.
o CSMLS Certification – national certification through the Canadian Society for Medical Laboratory Science (CSMLS).
• Successfully pass your chosen certification exam.
• Begin your career working in hospitals, medical laboratories, diagnostic clinics, or other healthcare environments.
Graduates may pursue opportunities in a variety of healthcare and laboratory environments, including:
• Hospitals and private diagnostic laboratories
• Physicians’ offices and medical clinics
• Pharmaceutical laboratories
• Insurance and medical assessment services
The MLAT diploma prepares graduates to support diagnostic testing, specimen collection, and laboratory operations as part of the healthcare team.
Applicants must have:
Admission Requirements
Ontario Secondary School Diploma or Mature Student Status
Trillium College Immunization Report; completed and signed by a medical professional.
Submission of a clear Police Check (CPIC) report with Vulnerable Sector Screening
Graduation Requirements
220 Placement hours completed
The best first step is to connect with a Career Coach. They can answer your questions, walk you through the program, and help you determine if the Medical Lab Assistant /Technician Diploma Program is the right path for you.